primary human follicle dp cells hfdpcs  (PromoCell)

Journal: bioRxiv

Article Title: Basement membrane components define the microenvironment of aggregated fibroblasts in the skin and support their aggregation in vitro

doi: 10.1101/2025.08.27.672757

Figure Lengend Snippet: (A) Schematic of the spheroid invasion assay. DP spheroids, each composed of 300 HFDPCs, were placed on an ECM gel layer and overlaid with an additional ECM gel layer. The spheroids were cultured and observed every 12 hours. (B) Phase-contrast images of DP spheroids cultured in collagen I gel (top) and Matrigel (bottom). The colored squares indicate regions magnified in the bottom right images. Scale bar: 100 µm. (C) Binary-masked images highlighting the spheroid area. (D) Line graphs showing the changes in spheroid area over time in collagen I gel (red) and Matrigel (green). (E) Box plots showing the relative spheroid area at 12 h, normalized to the initial time point (1 h). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Two-tailed unpaired t -tests were used: * p <0.05, ** p <0.01, and *** p <0.001. n = 9 wells. (F and G) Box plots showing the relative spheroid area with integrin blocking antibodies in collagen I gel (F) and Matrigel (G). The box shows the interquartile range with the median; whiskers extend to the 1.5 interquartile range. Statistical comparisons were performed using the Kruskal‒Wallis test followed by Dunn’s post hoc test. Adjusted p -values from the Benjamini‒Hochberg method are shown: * p <0.05, ** p <0.01, *** p <0.001. n = 9 wells pooled from 3 independent experiments.

Article Snippet: Primary human follicle DP cells (HFDPCs) were purchased from PromoCell (Heidelberg, Germany) and cultured in Follicle Papillae Growth Medium (PromoCell) at 37°C, 5% CO 2 and 100% humidity.

Techniques: Invasion Assay, Cell Culture, Two Tailed Test, Blocking Assay

Journal: Pharmaceutics

Article Title: In Vitro Biological Activity and In Vivo Human Study of Porcine-Placenta-Extract-Loaded Nanovesicle Formulations for Skin and Hair Rejuvenation

doi: 10.3390/pharmaceutics14091846

Figure Lengend Snippet: Effect of PPE on cell viability of ( A ) NHF; ( B ) HaCaT; ( C ) HFDPC. The cells were exposed to various concentrations (0–4000 µg/mL) of PPE for 24 h. Data are shown as mean ± SD ( n = 3). *, ** represent a significant difference from DMEM group (a negative control) at p -values of <0.05 and 0.001, respectively.

Article Snippet: Normal human foreskin fibroblast cells (NHF), human keratinocytes cells (HaCaT), and human follicle dermal papilla cells (HFDPC) were attained from the American-Type Culture Collection (ATCC) (Rockville, MD, USA).

Techniques: Negative Control

Journal: Pharmaceutics

Article Title: In Vitro Biological Activity and In Vivo Human Study of Porcine-Placenta-Extract-Loaded Nanovesicle Formulations for Skin and Hair Rejuvenation

doi: 10.3390/pharmaceutics14091846

Figure Lengend Snippet: Effect of PPE on the proliferation of ( A ) NHF, ( B ) HaCaT, and ( C ) HFDPC cells. Each cell was treated with various concentrations (0–2000 µg/mL) of PPE for different time intervals: 24 h ( ■ ), 48 h ( ■ ), and 72 h (☐). Data are presented as mean ± SD ( n = 3). * and ** represent the significant difference in % cell proliferation compared with the cell proliferation at 24 h at p -value < 0.05 and 0.001, respectively.

Article Snippet: Normal human foreskin fibroblast cells (NHF), human keratinocytes cells (HaCaT), and human follicle dermal papilla cells (HFDPC) were attained from the American-Type Culture Collection (ATCC) (Rockville, MD, USA).

Techniques:

Journal: Pharmaceutics

Article Title: In Vitro Biological Activity and In Vivo Human Study of Porcine-Placenta-Extract-Loaded Nanovesicle Formulations for Skin and Hair Rejuvenation

doi: 10.3390/pharmaceutics14091846

Figure Lengend Snippet: Effect of PPE on the aggregation of HFDPC cells after treatment for 72 h. HFDPC cells were treated with ( A ) DMEM (control group) and ( B ) 1000 µg/mL PPE. The images were photographed using an inverted microscope (10× objective lens, bright field).

Article Snippet: Normal human foreskin fibroblast cells (NHF), human keratinocytes cells (HaCaT), and human follicle dermal papilla cells (HFDPC) were attained from the American-Type Culture Collection (ATCC) (Rockville, MD, USA).

Techniques: Control, Inverted Microscopy